Description
The Real-Time Quantitative PCR System YR06248 – YR06249 is a sophisticated tool designed for scientists and researchers who require precise and sensitive PCR analysis. With state-of-the-art signal capture and spectrum separation technology, this system ensures excellent correction of multicolor crosstalk and consistent temperature uniformity. Its Peltier-based heating and cooling system supports a temperature range from 4°C to 100°C, delivering reliable results with high reproducibility. Available in 4-channel and 5-channel configurations, it excels in multiplex PCR applications, accommodating up to 4 or 5 targets simultaneously. The system is compatible with a variety of qPCR dyes and probes such as FAM, VIC, ROX, and Cy5, catering to diverse laboratory applications.
Market Price
The price of the YR06248 – YR06249 Real-Time Quantitative PCR System varies depending on the model and configuration chosen. This advanced equipment falls within the premium range of real-time PCR systems, reflecting its cutting-edge features and versatility. To receive an accurate quote and explore potential discounts as a Kalstein+ member, we invite you to request a quotation through the Kalstein Plus platform.
Frequently Asked Questions
- What sensitivity does the YR06248 PCR system offer? The system is capable of detecting a single gene copy, making it ideal for samples with low concentration.
- Is the system compatible with various PCR reagents? Yes, it supports a broad range of dyes and probes, including FAM, SYBR Green, VIC, ROX, and Cy5, allowing flexibility in multiplex assays.
- What maintenance is required for optimal performance? Regular maintenance includes cleaning optical components, calibrating temperature controls, and keeping software up to date.
Advantages and Disadvantages
Advantages:
- High sensitivity, with detection of just one gene copy.
- Compatibility with diverse dyes and probes.
- Multiplexing capability for up to 4 targets or 5 with the 5-channel model.
- Exceptional resolution and accuracy, suitable for complex samples.
- Precision heating and cooling facilitated by Peltier technology.
Disadvantages:
- High cost, which may be restrictive for laboratories with constrained budgets.
- Larger size may pose challenges in compact laboratory settings.
Real-World Use
The YR06248 – YR06249 PCR system is primarily deployed in genetic research laboratories, molecular diagnostics, and pharmaceutical studies. It plays a critical role in DNA and RNA quantification, gene expression analysis, genotyping, and studies of genetic variants. Clinically, it is instrumental in diagnosing infectious diseases, cancer, and genetic disorders. The system’s multiplex PCR capability enhances analysis efficiency by allowing for simultaneous evaluation of multiple biomarkers in a single run, conserving time and resources in busy labs.
Recommendations
For optimal results with the Real-Time Quantitative PCR System YR06248 – YR06249, it is recommended to utilize high-quality reagents compatible with the system. Regular calibration of temperature and optical systems is essential to maintain performance. Laboratories dealing with low-concentration or complex samples can significantly benefit from the system’s high precision and dynamic range. To explore specific pricing and options, users are encouraged to request a detailed quote via the Kalstein Plus platform.
Technical Specifications
Model | YR06248 | YR06249 |
Sample capacity | 8*0.1mL PCR strip*12 96*0.1mL individual tube 0.1mL PCR Plate |
|
Sample volume | 10-50 μL | |
Heating and Cooling method | Peltier | |
Maximum ramping rate | 6.0° C/s | |
Temperature range | 4-100 °C | |
Temperature accuracy | ± 0.2°C | |
Temperature uniformity | ±0.2°C@60°C ±0.3°C@95°C | |
Optical Detecting System | ||
Excitation light | 4pcs high-efficiency single color LED | 5pcs high-efficiency single color LED |
Detector | SiPMT | |
Detection method | Time-resolved real-time scanning | |
Excitation/Emission wavelengths | 455-650nm | 510-750nm |
Detection Channels | 4 (optional 5 channels) | |
Supported dyes | FAM/SYBR Green, VIC/JOE/HEX/TET, ROX/Texas Red, Cy5/LIZ (Optional- Cy5.5 for 5-channel model) | |
Multiplexing | Up to 4 targets (optional 5 targets for 5-channel model) | |
Sensitivity | 1 copy gene | |
Resolution | 1.33-fold copies difference in single-plex reaction | |
Dynamic range | 10 orders of magnitude |